An RNA transcription-based amplification technique (NASBA) for the detection of viable Salmonella enterica
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چکیده
منابع مشابه
Evaluation of Nucleic Acid Sequence Based Amplification (NASBA) and Reverse Transcription Polymerase Chain Reaction for Detection of Coxsackievirus B3 in Cell Culture and Animal Tissue Samples
Enteroviruses are the causative agents of a number of diseases in humans. Group B coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. Genomic data of enteroviruses has allowed developing new molecular approaches such as Nucleic Acid Sequence Based Amplification (NASBA) for detection of such viruses. In this study, coxsackievirus B3 (CVB3) was de...
متن کاملMolecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA.
Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato leafroll virus (PLRV). During amplification, the probe anneals to the antisense RNA amplicon genera...
متن کاملevaluation of nucleic acid sequence based amplification (nasba) and reverse transcription polymerase chain reaction for detection of coxsackievirus b3 in cell culture and animal tissue samples
enteroviruses are the causative agents of a number of diseases in humans. group b coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. genomic data of enteroviruses has allowed developing new molecular approaches such as nucleic acid sequence based amplification (nasba) for detection of such viruses. in this study, coxsackievirus b3 (cvb3) was de...
متن کاملDetection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
متن کاملFabrication of Plasmonic Substrates Using Facile Technique of Spin-Coating for Salmonella Bacteria Detection
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2000
ISSN: 0266-8254,1365-2673
DOI: 10.1046/j.1472-765x.2000.00670.x